Three-Dimensional Modelling inside a Differential Pressure Laminar Flow Bioreactor Filled with Porous Media
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Hindawi Publishing Corporation BioMed Research International
Saturday, February 21, 2015

A three-dimensional computational fluid dynamics- (CFD-) model based on a differential pressure laminar flow bioreactor
prototype was developed to further examine performance under changing culture conditions. Cell growth inside scaffolds was
simulated by decreasing intrinsic permeability values and led to pressure build-up in the upper culture chamber. Pressure release
by an integrated bypass system allowed continuation of culture. The specific shape of the bioreactor culture vessel supported a
homogenous flow profile and mass flux at the scaffold level at various scaffold permeabilities. Experimental data showed an increase
in oxygen concentration measured inside a collagen scaffold seeded with human mesenchymal stem cells when cultured in the
perfusion bioreactor after 24 h compared to static culture in a Petri dish (dynamic: 11% O2 versus static: 3% O2). Computational
fluid simulation can support design of bioreactor systems for tissue engineering application.

Author Name: 
Birgit Weyand
Meir Israelowitz
James Kramer
Christian Bodmer
Mariel Noehre
Sarah Strauss
Elmar Schmälzlin
Christoph Gille
Herbert P. von Schroeder
Kerstin Reimers
Peter M. Vogt
Author Company: 
Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, Carl-Neuberg-Straße 1, 30625 Hannover, Germany
Biomimetics Technologies Inc., 191 Ellis Avenue, Toronto, ON, Canada M6S 2X4
8 Selden Street, Newton, MA 02468, USA
Habsburger Allee 56 B, 76767Hagenbach, Germany
Colibri Photonics GmbH, Am M¨uhlenberg 11, 14476 Potsdam, Germany
TorontoWesternHospital, University of Toronto, East Wing 2nd Floor, 399 Bathurst Street, Toronto,ON, Canada M5T 2S8